Alteration of host cell ubiquitination by the intracellular bacterial pathogen Coxiella burnetii

The intracellular bacterial agent of Q fever, Coxiella burnetii, replicates within a phagolysosome-like parasitophorous vacuole (PV) in human macrophages and delivers effector proteins to the host cytosol via a Dot/Icm type IV secretion system (T4SS). T4SS effectors are critical for PV formation and prevention of host cell death that allows sufficient time for bacterial replication. Recruitment of ubiquitin-related components to the C. burnetii PV is also predicted to be involved in PV formation and bacterial replication and is likely controlled by effector proteins. In this study, we assessed the role of the Dot/Icm T4SS in regulating ubiquitination by comparing subcellular localization of ubiquitinated proteins between cells infected with wild type C. burnetii and a mutant that lacks a functional T4SS. Fluorescence microscopy showed ubiquitinated proteins surrounding wild type C. burnetii PV but not phagosomes harboring T4SS-defective organisms. Immunoblot analysis showed altered ubiquitinated protein profiles throughout infection, suggesting C. burnetii impacts post-translational modification of host cell and/or bacterial proteins. Future studies will determine how T4SS-mediated recruitment of ubiquitinated proteins impacts C. burnetii-host cell interactions and eventual development of disease

Gender disparity in South Asia : comparisons between and within countries

Using data assembled from the Demographic Health Surveys of over 50 countries and from the National Family Health Surveys of individual states in India, the authors create a new data set of comparable indicators of gender disparity. They establish three findings: 1) As is by now well-known, the level of gender disparities in health and education outcomes for girls in South Asia is the highest in the world. 2) Even within South Asia, and within India or Pakistan, there are huge variations in gender disparity. Differences in gender disparity among Indian states or among provinces in Pakistan are typically greater than those among the world's nations. The ratio of female to male child mortality in one Indian state (Haryana) is worse than in any country in the world, although in another state (Tamil Nadu) it is lower than in all but three countries. 3) Across and within the set of developing nations, gender disparity is not only a phenomenon of poverty. There is almost no correlation between per capita income and the gender disparities in health and education outcomes. So although absolute levels of health and education outcomes for girls are strongly related to economic conditions, the disparities between outcomes for girls and boys are not. Understanding what causes such great gender disparity within South Asia is the next pressing question for researchers.Public Health Promotion,Early Childhood Development,Early Child and Children's Health,Health Monitoring&Evaluation,Health Economics&Finance,Health Economics&Finance,Early Child and Children's Health,Adolescent Health,Early Childhood Development,Health Monitoring&Evaluation

Effect of a Sliding Scale Vitamin D Supplementation Protocol on 25(OH)D Status in Elite Athletes with Spinal Cord Injury

Recent studies suggest that a substantial proportion of elite athletes with SCI (spinal cord injury) have insufficient 25(OH)D status which may be associated with decreased muscle strength. This study: 1) examined the effects of a 16-week sliding scale Vitamin D supplementation protocol on 25(OH)D concentration and 2) determined whether subsequent 25(OH)D status impacts muscle performance in elite athletes with SCI. Thirty-four members of the US Olympic Committee Paralympic program, and the Canadian Wheelchair Sports Association from outdoor sports participated. Serum 25(OH)D concentrations, lifestyle and dietary factors were assessed during the Winter and Spring. Participants were assigned a 16-week sliding scale vitamin D3 (cholecalciferol) (KleanAthlete Brand) supplementation protocol based on initial 25(OH)D levels. Participants with deficient 25(OH)D (\u3c50 nmol/L) status received 50,000 IU/wk. for 8 wks., and participants with insufficient status (50-75 nmol/L) received 35,000 IU/week for 4 weeks followed by a maintenance dosage of 15,000 IU/wk. Participants with sufficient status (\u3e75nmol/L) received the maintenance dosage of 15,000 IU/wk. 25 (OH)D concentrations increased significantly after supplementation (p \u3c.001; 66.3 + 24.3 nmol/L; 111.3 + 30.8 nmol/L) for Winter and Spring, respectively. 26% of athletes had sufficient 25(OH)D concentrations prior to supplementation, and 91% had sufficient concentrations post supplementation. 62% of participants improved handgrip strength post supplementation. No change in 20-meter wheelchair sprint performance time was observed. The 16-week sliding scale supplementation protocol used in the current study is effective for achieving sufficient vitamin D concentrations during the winter months in elite athletes with SCI

Effects of Pre-Exercise Ice Slurry Ingestion on Physiological and Perceptual Measures in Athletes with Spinal Cord Injuries

Athletes with spinal cord injuries (SCI) have an impaired ability to thermoregulate during exercise, leading to an increased core temperature (Tcore) due to a decrease in sweat response. Elevated core temperature may result in premature onset of fatigue and decreased athletic performance. Therefore, precooling techniques that decrease Tcore before exercise may increase the storage capacity for metabolic heat production, thereby improving exercise performance. The purpose of this study was to investigate the effects of pre-exercise ice slurry ingestion as a precooling method in elite athletes with SCI during a match simulation. Employing a field-based, counterbalanced-design, subjects were administered 6.8 g/kg of room temperature (PLB) or ice slurry (IS) beverage during a 20 minute precooling period, before engaging in a 50 and 60 minute on-court training session on day 1 and 2, respectively. Physiological measures, including Tcore and heart rate, and perceptual measures including gastrointestinal and thermal comfort, and rating of perceived exertion, were monitored throughout precooling (minutes 10, 20) and exercise (minutes 10-60). IS had a large effect on Tcore at the midpoint of exercise on day 1 (minute 30) (ES = 0.73) and 2 (minute 40) (ES = 1.17). Independent samples T-tests revealed significant differences in the perception of thermal comfort between IS and PLB at the midpoint of exercise on both day 1 (minute 30) (P = 0.04) and 2 (minute 40) (P = 0.05), indicating that IS helped subjects to feel cooler during exercise. In conclusion, pre-exercise ice slurry ingestion provides an effective means for delaying an increase in Tcore in athletes with SCI

Energy and Nutrient Issues in Athletes with Spinal Cord Injury: Are They at Risk for Low Energy Availability?

Low energy availability (LEA) and nutrient intake have been well studied in able-bodied athletes, but there is a lack of research examining these issues amongst athletes with spinal cord injury (SCI). To date, there have been no studies that have examined energy availability (EA) amongst this population. Furthermore, athletes with SCI may experience unique challenges around nutrition that may increase their risk of LEA. This review will evaluate the literature and assess whether this population is at risk for LEA. Due to the limited research on this topic, sedentary individuals with SCI and para athletes were also included in this review. Review of the current literature suggests that athletes with SCI may be at an increased risk for LEA. While research examining EA and risk of LEA in athletes with SCI is lacking, the number of athletes with SCI continues to increase; therefore, further research is warranted to assess nutrient and energy needs and their risk to this population

Defining the role and experiences of service-learning faculty : a qualitative study at The University of Texas at Austin

textOver the past two decades researchers have analyzed motivating factors and institutional barriers that influenced a professor's initial decision to utilize a service-learning pedagogy. The majority of this research has been quantitative in nature, surveying faculty members' initial attitudes around service-learning. However, the extant literature fails to qualitatively examine the experiences of faculty members who successfully integrate service-learning, especially at a public research institution with civic-engaged mission. Because a public institution relies upon a critical mass of faculty members to support its civic engagement mission, this study focused on explaining the lived experience of exemplar professors in service-learning to understanding their motivations, barriers, and experiences. Faculty members are important to study because service-learning is a form of community engagement that cannot happen without sustainable efforts from professors. Moreover, students and communities cannot derive the benefits of service-learning, nor can civically minded institutions achieve their goal, if faculty members do not incorporate service-learning into their classrooms. Thus, the purpose of this qualitative phenomenological study is to understand the experiences of service-learning faculty members at a four-year public research institution where community engagement is a stated priority. Utilizing a recently developed faculty engagement model (Demb & Wade, 2012) as the guiding theoretical framework, this research study seeks to understand the lived experience of faculty members at The University of Texas at Austin by inquiring 1) how faculty members implement meaningful community engagement through their service-learning classes, 2) how service-learning may shape a faculty members' professional and personal identity at a research institution, and 3) how service-learning fits into faculty members' larger scholarship agenda.Educational Administratio

25(OH)D Status of Elite Athletes with Spinal Cord Injury Relative to Lifestyle Factors

Background: Due to the potential negative impact of low Vitamin D status on performance-related factors and the higher risk of low Vitamin D status in Spinal Cord Injury (SCI) population, research is warranted to determine whether elite athletes with SCI have sufficient 25(OH)D levels. The purposes of this study were to examine: (1) the seasonal proportion of vitamin D insufficiency among elite athletes with SCI; and (2) to determine whether lifestyle factors, SCI lesion level, and muscle performance/function are related to vitamin D status in athletes with SCI. Methods: Thirty-nine members of the Canadian Wheelchair Sports Association, and the US Olympic Committee Paralympic program from outdoor and indoor sports were recruited for this study. Dietary and lifestyle factors, and serum 25(OH)D concentrations were assessed during the autumn (October) and winter (February/March). An independent t-test was used to assess differences in 25(OH)D status among seasons, and indoor and outdoor sports in the autumn and winter, respectively. Results: Mean ± SD serum 25(OH)D concentration was 69.6 ± 19.7 nmol/L (range from 30 to 107.3 nmol/L) and 67.4 ± 25.5 nmol/L (range from 20 to 117.3 nmol/L) in the autumn and winter, respectively. In the autumn, 15.4% of participants were considered vitamin D deficient (25(OH)D \u3c 50 nmol/L) whereas 51.3% had 25(OH)D concentrations that would be considered insufficient (\u3c80 nmol/L). In the winter, 15.4% were deficient while 41% of all participants were considered vitamin D insufficient. Conclusion: A substantial proportion of elite athletes with SCI have insufficient (41%–51%) and deficient (15.4%) 25(OH)D status in the autumn and winter. Furthermore, a seasonal decline in vitamin D status was not observed in the current study

Risk of Low Energy Availability in National and International Level Paralympic Athletes: An Exploratory Investigation

(1) Background: The purpose of this study was to examine the symptoms of low energy availability (LEA) and risk of relative energy deficiency in sport (RED-S) symptoms in para-athletes using a multi-parameter approach. (2) Methods: National level para-athletes (n = 9 males, n = 9 females) completed 7-day food and activity logs to quantify energy availability (EA), the LEA in Females Questionnaire (LEAF-Q), dual energy X-ray absorptiometry (DXA) scans to assess bone mineral density (BMD), and hormonal blood spot testing. (3) Results: Based on EA calculations, no athlete was at risk for LEA (females \u3c 30 kcal·kg−1 FFM·day−1; and males \u3c 25 kcal·kg−1 FFM·day−1; thresholds for able-bodied (AB) subjects). Overall, 78% of females were “at risk” for LEA using the LEAF-Q, and 67% reported birth control use, with three of these participants reporting menstrual dysfunction. BMD was clinically low in the hip (\u3c−2 z-score) for 56% of female and 25% of male athletes (4) Conclusions: Based on calculated EA, the risk for RED-S appears to be low, but hormonal outcomes suggest that RED-S risk is high in this para-athlete population. This considerable discrepancy in various EA and RED-S assessment tools suggests the need for further investigation to determine the true prevalence of RED-S in para-athlete populations

Reducing Aggressive Responses to TCAS: Evaluation of a TCAS Training Program

The Traffic alert and Collision Avoidance System (TCAS) is an aircraft collision avoidance system designed to prevent mid-air collisions. While responding to a TCAS advisory is generally the safe course of action, instances of overly aggressive responses have resulted in injuries to crew members and passengers as well as disruptions in air traffic operations. However, current training standards do not address the need to mitigate overly aggressive responses. This paper details the design and evaluation of a training program for TCAS which incorporated a learning objective related to mitigating aggressive responses to advisories. The impact of the training program was evaluated by comparing the results of two flight simulator experiments. These experiments examined “trained” and “untrained” pilot responses to TCAS advisories in an integrated flight deck-Air Traffic Control simulator. Overall, the training program had a significant impact on the pilots’ behavior and aggressive responses to TCAS advisories were decreased

Pollinator Plants for Stormwater Management

Final project for PLSC 460: Application of Knowledge in Plant Science (Spring 2021). University of Maryland, College Park.The group of students provided recommendations to M-NCPPC Parks to develop a pollinator plant selection guide for three stormwater management facilities: a parking lot island, a building foundation planting, and an open field. The selection guide included site information for three types of plantings (proper use of stormwater control measures and viewpoints) and plant use facts (environmental metrics and other characteristics) and pictures.Prince George's County Department of Parks & Recreation (PGDPR